Cdh5-2A-CreERT2

Nomenclature

C57BL/6Smoc-Cdh5em1(2A-CreERT2-WPRE-polyA)Smoc

Cat. NO.

NM-KI-200173

Strain State

Repository Live

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Gene Summary

Gene Symbol
Cdh5

Model Description

A 2A-CreERT2-WPRE-polyA expression cassette was knocked into the Cdh5 gene stop codon site.
Research Application:CDH5(vascular endothelial cadherin ,VEC) is expressed in the vasculature. VEC-Cre will mark both endothelial cells and blood cells in lineage tracing experiments.

Validation Data

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Fig. 1 CreERT2-mediated recombination in the aorta of Cdh5CreERT2 /+; Rosa26tdTomato/+ mouse. TdTomato(red) expression can be detected in the arterial endothelial cells of Cdh5CreERT2 /+; Rosa26tdTomato/+ mouse after tamoxifen treatment.

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Fig. 2 CreERT2-mediated recombination in the spinal cord of Cdh5CreERT2 /+; Rosa26tdTomato/+ mouse. TdTomato(red) expression can be detected in the endothelial cells derived from spinal cord of Cdh5CreERT2 /+; Rosa26tdTomato/+ mouse after tamoxifen treatment.

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Fig. 3 CreERT2-mediated recombination in the cerebellum of Cdh5CreERT2 /+; Rosa26tdTomato/+ mouse. TdTomato(red) expression can be detected in some cells of cerebellum derived from Cdh5CreERT2 /+; Rosa26tdTomato/+ mouse after tamoxifen treatment.


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Fig. 4 Detection of tdTomato(red) in various tissues of Cdh5CreERT2/+; Rosa26tdTomato/+ mice. CreERT2 mediated recombination can be detected in the brain, pituitary gland, eyes, heart, lung, liver, submandibular gland, kidney, pancreas, testis, muscle, intestine, colon, spleen, thymus and brown fat. TdTomato expression can not be observed in the epidermis. (For more detailed information please contact our technical advisor.)



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