hAPOE2

Nomenclature

C57BL/6Smoc-Apoetm4(hAPOE2)Smoc

Cat. NO.

NM-HU-190013

Strain State

Repository Live

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Gene Summary

Gene Symbol
Apoe

Synonyms

Apo-E; AI255918

NCBI ID

11816

MGI ID

88057

Ensembl ID

ENSMUSG00000002985

Pubmed

Apoe

Human Ortholog

APOE

Model Description

This APOE2 humanized mouse model was established by replacing the mouse Apoe gene coding exons and introns with the human APOE2 corresponding sequence by homologous recombination.
Research Application：Immune-related

Validation Data

Generation strategy

Apoe2-1.jpg

Fig1.   Schematic representation of gene targeting at the Apoe locus.

Validation data

Apoe2-2.png

Fig2. Genotyping of WT , KO &  Humanized mice by PCR.

Primer P1/2 Wild type = 342 bp

Primer P3/4 mutant = 332 bp

M：100bp Plus DNA ladder.

Apoe2-4.png

Fig3. Western blot analysis of  APOE2 protein levels in  WT、KO & humanized mice liver.  

APOE anti-body：Sc-13521 (santa cruz)

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