hMAPT

The endogenous mouse Mapt gene was replaced by human MAPT gene. While hMAPT(2)(Stock No.NM-HU-200257) mice function similarly to hMAPT mice,for more detailed information please contact our technical advisor.

Fig.1 Detection of MAPT expression in cerebellum by RT-PCR. 

Wild type: only one band at 200 bp with primers F1/R1 (mMapt); 

Homozygous: only one band at 181 bp with primers F2/R2 (hMAPT); 

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

Fig 2. Detection of mouse MAPT expression in Brain in hMAPT knockin mice by IHC. 

Brains were collected from wild-type mice (+/+) and homozygous hMAPT mice (HO), and analyzed by IHC with anti-human MAPT antibody. 

Abbr. HO/HO, homozygous; +/+, wild type. 

Note. The anti-mouse MAPT Antibody cross-reacted with mouse MAPT and humanized MAPT.

Fig 3. Detection of human MAPT expression in Brain in hMAPT knockin mice by IHC. 

Brains were collected from wild-type mice (+/+) and homozygous hMAPT mice (HO), and analyzed by IHC with anti-human MAPT antibody. 

Abbr. HO/HO, homozygous; +/+, wild type. 

Note. The anti-human MAPT Antibody cross-reacted with mouse MAPT and humanized MAPT.

Fig 4. Detection of human MAPT expression in Brain in hMAPT knockin mice by IF. 

Brains were collected from wild-type mice (+/+) and homozygous hMAPT mice (HO), and analyzed by IF with anti-human MAPT antibody. 

Abbr. HO/HO, homozygous; +/+, wild type. 

Note. The anti-human MAPT Antibody cross-reacted with mouse MAPT and humanized MAPT.