hEPCAM

Nomenclature

C57BL/6Smoc-EpCAMtm1(hEpCAM)Smoc

Cat. NO.

NM-HU-210032

Strain State

Sperm cryopreservation

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Gene Summary

Gene Symbol
Epcam

Model Description

The endogenous mice Epcam gene was completely or partially replaced by human EPCAM gene via ESC targeting.
Research Application:inmmue therapy;drug screening

Validation Data

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Fig1. Analysis of mouse EPCAM and human EPCAM protein expression in the large intestine by IHC. Both the EPCAM humanized heterozygous mouse and wild type express mouse EPCAM in the mucosal epithelial membranes, and the humanized heterozygous mouse  expresses human EPCAM in the crypts significantly.

2.pngFig2. Analysis of mouse EPCAM and human EPCAM protein expression in the small intestine by IHC. Both the EPCAM humanized heterozygous mouse and wild type express mouse EPCAM in the mucosal epithelial membranes, and the humanized heterozygous mouse  expresses human EPCAM in the crypts significantly.

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Fig3. Detection of human EpCAM expression in WT C57 and homozygous hEpCAM mice by IHC. Small intestine were collected from wild-type mice (+/+) and homozygous hEpCAM mice (HO/HO), and analyzed by IHC with anti-human EpCAM antibody. 


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